SR-BI antibody detects SR-BI protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SR-BI protein stained by SR-BI antibody (GTX113645) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

HepG2 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SR-BI antibody (GTX113645) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SR-BI antibody detects SR-BI protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SR-BI protein stained by SR-BI antibody (GTX113645) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using SR-BI(GTX113645) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SR-BI antibody detects SR-BI protein by western blot analysis.
A. 50 μg rat liver extract
7.5% SDS-PAGE
SR-BI antibody (GTX113645) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SR-BI antibody detects SR-BI protein at cytosol and weak membrane on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
SR-BI antibody (GTX113645) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the 2022 in Mol Med. PMID: 35236285