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STAT3 (phospho Ser727) antibody

Orthogonal
Anti-STAT3 (phospho Ser727) antibody used in Western Blot (WB). GTX50120
Anti-STAT3 (phospho Ser727) antibody used in Western Blot (WB). GTX50120
Anti-STAT3 (phospho Ser727) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50120
Anti-STAT3 (phospho Ser727) antibody used in IHC (Paraffin sections) (IHC-P). GTX50120

Cat. No. GTX50120

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all STAT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-R) derived from human STAT3.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 3 , ADMIO , ADMIO1 , APRF , HIES

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-STAT3 (phospho Ser727) antibody used in Western Blot (WB). GTX50120

GTX50120 WB Image

WB analysis of extracts from HeLa cells treated with IFNa or calf intestinal phosphatase (CIP) using GTX50120 STAT3 (phospho Ser727) antibody.

Anti-STAT3 (phospho Ser727) antibody used in Western Blot (WB). GTX50120

GTX50120 WB Image

WB analysis of extracts from HeLa and HepG2 cells untreated or treated with PMA using GTX50120 STAT3 (phospho Ser727) antibody.

Anti-STAT3 (phospho Ser727) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50120

GTX50120 ICC/IF Image

Orthogonal

ICC/IF analysis of methanol-fixed HeLa cells using GTX50120 STAT3 (phospho Ser727) antibody.

Anti-STAT3 (phospho Ser727) antibody used in IHC (Paraffin sections) (IHC-P). GTX50120

GTX50120 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50120 STAT3 (phospho Ser727) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399