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STUB1 antibody [N1C3]

Anti-STUB1 antibody [N1C3] used in Western Blot (WB). GTX122827
Anti-STUB1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX122827
Anti-STUB1 antibody [N1C3] used in Western Blot (WB). GTX122827
Anti-STUB1 antibody [N1C3] used in Western Blot (WB). GTX122827

Cat. No. GTX122827

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human STUB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178641

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

STIP1 homology and U-box containing protein 1 , CHIP , HSPABP2 , NY-CO-7 , SCA48 , SCAR16 , SDCCAG7 , UBOX1

Cellular Localization

Cytoplasm

Background

STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation (Min et al., 2008 [PubMed 18411298]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-STUB1 antibody [N1C3] used in Western Blot (WB). GTX122827

GTX122827 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX122827 diluted at 1:10000

Anti-STUB1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX122827

GTX122827 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using STUB1(GTX122827) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-STUB1 antibody [N1C3] used in Western Blot (WB). GTX122827

GTX122827 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with STUB1 antibody [N1C3] (GTX122827) diluted at 1:10000.

Anti-STUB1 antibody [N1C3] used in Western Blot (WB). GTX122827

GTX122827 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with STUB1 antibody [N1C3] (GTX122827) diluted at 1:10000.

REFERENCE

There are currently no references for STUB1 antibody [N1C3] (GTX122827). Be the first to share your publications with this product.

REVIEW

STUB1 antibody [N1C3] Cat. No. GTX122827

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-Oct-2014
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 20μg
Blocking : 1% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 2Hr
Lane Description : Lane 1: BT-474 cells, Lane 2: HCC1937 cells, Lane 3: Hs578T cells, Lane 4: MCF7 cells, Lane 5: MDA-MB-231 cells, Lane 6: MDA-MB-361 cells, Lane 7: MDA-MB-453 cells, Lane 8: T-47D cells, Lane 9: ZR-75-1 cells, Lane 10: HeLa cells, Lane 11: U2OS cells, Lane 12: 293T cells
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169