Wild-type (WT) and SUCLG2 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG2 antibody (GTX107002) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG2 antibody (GTX107002) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (20 μg of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX107002 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
10% SDS PAGE
GTX107002 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SUCLG2 antibody detects SUCLG2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: SUCLG2 protein stained by SUCLG2 antibody (GTX107002) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SUCLG2 antibody detects SUCLG2 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
SUCLG2 antibody (GTX107002) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal Dis Model Mech in 2014. PMID: 24271779