Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (GTX637784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (GTX637784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (GTX637784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

SUN1 antibody [HL1947] detects SUN1 protein at nuclear envelope by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SUN1 stained by SUN1 antibody [HL1947] (GTX637784) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (GTX637784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (GTX637784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SUN1 antibody [HL1947] detects SUN1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SUN1 stained by SUN1 antibody [HL1947] (GTX637784) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min