Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using SUV39H1(GTX112263) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUV39H1 antibody [N3C3] (GTX112263) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SUV39H1 antibody [N3C3] detects SUV39H1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SUV39H1 stained by SUV39H1 antibody [N3C3] (GTX112263) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.