GTX65630-pro Salmonella typhimurium Flagellin protein activates TLR5 in vivo. Mice WT are injected i.v. with indicated doses of Flagellin protein. After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10μg of Flagellin protein, but no expression of CCL20 and IL-6 could be observed (not shown).