Immunofluorescence analysis of methanol-fixed A549, using SerpinB3(GTX114739) antibody at 1:500 dilution.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SerpinB3 antibody [N2C3] (GTX114739) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SerpinB3 antibody [N2C3] detects SerpinB3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
SerpinB3 stained by SerpinB3 antibody [N2C3] (GTX114739) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SerpinB3 antibody [N2C3] (GTX114739) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.