Shh antibody [HL2076] detects Shh protein at cell membrane by immunofluorescent analysis.
Sample: RT-4 cells were fixed in ice-cold MeOH for 5 min.
Green: Shh stained by Shh antibody [HL2076] (GTX637984) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Shh antibody [HL2076] (GTX637984) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Shh antibody [HL2076] (GTX637984) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Shh antibody [HL2076] (GTX637984) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.