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Smoothened homolog antibody [HL2975]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-Smoothened homolog antibody [HL2975] used in Western Blot (WB). GTX640362
Anti-Smoothened homolog antibody [HL2975] used in Western Blot (WB). GTX640362
Anti-Smoothened homolog antibody [HL2975] used in Western Blot (WB). GTX640362
Anti-Smoothened homolog antibody [HL2975] used in Western Blot (WB). GTX640362

Cat. No. GTX640362

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2975

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Smoothened homolog products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Product Note

This antibody was raised against human Smoothened homolog Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment encompassing a sequence within the Intracellular domain of human Smoothened homolog. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

smoothened, frizzled class receptor , CRJS , FZD11 , Gx , SMOH

Cellular Localization

Membrane,Cell projection, cilium

Background

The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Smoothened homolog antibody [HL2975] used in Western Blot (WB). GTX640362

GTX640362 WB Image

KO/KD Orthogonal

Boiled and unboiled wild-type (WT) and Smoothened homolog knockout (KO) T-47D cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Smoothened homolog antibody [HL2975] used in Western Blot (WB). GTX640362

GTX640362 WB Image

Overexpression

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Smoothened homolog antibody [HL2975] used in Western Blot (WB). GTX640362

GTX640362 WB Image

Orthogonal

Boiled and unboiled mouse brain extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-Smoothened homolog antibody [HL2975] used in Western Blot (WB). GTX640362

GTX640362 WB Image

Orthogonal

Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Smoothened homolog antibody [HL2975] (GTX640362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. (WCE: whole cell extract; ME: membrane extract)

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
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Custom Conjugations