A431 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

A431 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sodium/Potassium ATPase alpha 1 antibody [HL114] detects Sodium/Potassium ATPase alpha 1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Sodium/Potassium ATPase alpha 1 stained by Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sodium/Potassium ATPase alpha 1 antibody [HL114] detects Sodium/Potassium ATPase alpha 1 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Sodium/Potassium ATPase alpha 1 stained by Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Drosophila tissue extract (5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sodium/Potassium ATPase alpha 1 antibody [HL114] detects Sodium/Potassium ATPase alpha 1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Sodium/Potassium ATPase alpha 1 stained by Sodium/Potassium ATPase alpha 1 antibody [HL114] (GTX635461) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the 2022 in Nat Commun. PMID: 35618735