This antibody recognizes an epitope between amino acid residues 496-506 of lamb kidney sodium/potassium ATPase.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Sheep
Immunogen
Lamb kidney alpha 1 sodium/potassium ATPase.
Purification
Protein A purified
Conjugation
Unconjugated
RRID
AB_384891
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
ICC/IF analysis of HeLa cells using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:100 for at least 1 hour at room temperature
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GTX22872 WB Image
WB analysis of 25 ug of rat kidney (lane 1) and mouse kidney (lane 2) using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Dilution : 1:1000
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GTX22872 IHC-P Image
IHC-P analysis of human kidney tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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GTX22872 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
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GTX22872 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX22872 Sodium/Potassium ATPase alpha 1 antibody [M8-P1-A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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