WB analysis of 25 ug of Hela cell lysates using GTX22808 Spectrin beta 1 antibody [4C3].
Dilution : 1:1000

ICC/IF analysis of Hela cells using GTX22808 Spectrin beta 1 antibody [4C3]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

ICC/IF analysis of A431 cells using GTX22808 Spectrin beta 1 antibody [4C3]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

FACS analysis of NIH/3T3 cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

FACS analysis of SH-SY5Y cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

IHC-P analysis of human prostate carcinoma tissue using GTX22808 Spectrin beta 1 antibody [4C3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:20

IHC-P analysis of mouse skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:20

FACS analysis of Hela cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

IHC-P analysis of human skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200