Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX114783 diluted at 1:500

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Spermine synthase antibody [N3C3] (GTX114783) diluted at 1:500.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: JurKat
10% SDS PAGE
GTX114783 diluted at 1:1000

Immunofluorescence analysis of methanol-fixed HeLa, using Spermine synthase(GTX114783) antibody at 1:200 dilution.