Name: Streptavidin (HRP)
Brand: GeneTex
SKU: GTX27403
Price: 179.00 USD

Streptavidin (HRP)

Cat. No. GTX27403

GTX27403 WB Image

WB analysis of human IL-7 protein using biontin-conjugated anti-IL-7 antibody followed by GTX27403 Streptavidin (HRP).
Loading : 50 ng
Dilution : 1:40000

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GTX27403 Dot Image

Dot blot analysis of BSA with (row A) and without (row B) biotin conjugation using GTX27403 Streptavidin (HRP).
Lane 1 : 100 ng
Lane 2 : 33.33 ng
Lane 3 : 11.11 ng
Lane 4 : 3.7 ng
Lane 5 : 1.23 ng
Dilution : 1 μg/mL

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GTX27403 WB Image

WB analysis of GST protein using biotin-conjugated anti-GST antibody followed by GTX27403 Streptavidin (HRP).
Loading : 0.05 μg
Dilution : 1:40000

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GTX27403 WB Image

WB analysis of human IL-7 protein using Human IL-7 Biotin conjugated antibody followed by GTX27403 Streptavidin (HRP).
Loading : 50 ng
Dilution : 1:1000

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GTX27403 ELISA Image

Detection of Rabbit anti Mouse TNFα:biotin (GTX74045) in a sandwich ELISA with recombinant murine TNFα as the antigen and Rat anti Mouse TNFα as the coating antibody using Streptavidin:HRP (GTX27403)

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GTX27403 WB Image

Detection of Mouse anti histidine tag:biotin (GTX74967) in a Western blot analysis of a HIS tagged protein using Streptavidin:HRP (GTX27403)

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Applications

WB IHC-P IHC-Fr Dot ELISA IM

Summary

Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors.