Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Survivin(GTX100052) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using survivin (GTX100052) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Survivin antibody detects Survivin protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Survivin protein stained by Survivin antibody (GTX100052) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Immunoprecipitation of Survivin protein from 293T whole cell extracts using 5 μg of Survivin antibody (GTX100052).
Western blot analysis was performed using Survivin antibody (GTX100052).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Survivin antibody detects Survivin protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
12% SDS-PAGE
Survivin antibody (GTX100052) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Survivin antibody detects Survivin protein at nucleus by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human breast cancer.
Survivin stained by Survivin antibody (GTX100052) diluted at 1:100.
Antigen Retrieval: EDTA buffer, 30 min