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Survivin antibody

Orthogonal
Anti-Survivin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100052
Anti-Survivin antibody used in IHC (Paraffin sections) (IHC-P). GTX100052
Anti-Survivin antibody used in Western Blot (WB). GTX100052
Anti-Survivin antibody used in Immunoprecipitation (IP). GTX100052
Anti-Survivin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100052
Anti-Survivin antibody used in IHC (Paraffin sections) (IHC-P). GTX100052

Cat. No. GTX100052

Reference 16
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Survivin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

293T , HeLa

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Survivin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241366

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

baculoviral IAP repeat containing 5 , API4 , EPR-1

Cellular Localization

Cytoplasm , Nucleus , Centromere

Background

Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that, in humans, is encoded by the BIRC5 gene.This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Survivin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100052

GTX100052 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Survivin(GTX100052) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Survivin antibody used in IHC (Paraffin sections) (IHC-P). GTX100052

GTX100052 IHC-P Image

Survivin antibody detects Survivin protein at nucleus by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human breast cancer.
Survivin stained by Survivin antibody (GTX100052) diluted at 1:100.
Antigen Retrieval: EDTA buffer, 30 min

Anti-Survivin antibody used in Western Blot (WB). GTX100052

GTX100052 WB Image

Survivin antibody detects Survivin protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
12% SDS-PAGE
Survivin antibody (GTX100052) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Survivin antibody used in Immunoprecipitation (IP). GTX100052

GTX100052 IP Image

Immunoprecipitation of Survivin protein from 293T whole cell extracts using 5 μg of Survivin antibody (GTX100052).
Western blot analysis was performed using Survivin antibody (GTX100052).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Survivin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100052

GTX100052 ICC/IF Image

Survivin antibody detects Survivin protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Survivin protein stained by Survivin antibody (GTX100052) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Survivin antibody used in IHC (Paraffin sections) (IHC-P). GTX100052

GTX100052 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using survivin (GTX100052) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Mikami M et al. Mol Cells 2022; 45 (12) : 886-895 RUNX1-Survivin Axis Is a Novel Therapeutic Target for Malignant Rhabdoid Tumors.
Hattori EY et al. Commun Biol 2022; 5 (1) : 939 A RUNX-targeted gene switch-off approach modulates the BIRC5/PIF1-p21 pathway and reduces glioblastoma growth in mice.
Application : WB
Reactivity : Human
Lin TY et al. Phytomedicine 2021; 80 : 153384 Functional proteomic analysis reveals that fungal immunomodulatory protein reduced expressions of heat shock proteins correlates to apoptosis in lung cancer cells.
Application : WB
Reactivity : Mouse
Hueng-Chuen Fan et al. Int J Mol Sci 2020; 21 (22) Dehydroxyhispolon Methyl Ether, A Hispolon Derivative, Inhibits WNT/β-Catenin Signaling to Elicit Human Colorectal Carcinoma Cell Apoptosis.
Application : WB
Reactivity : Human
Su WL et al. Cancer Biomark 2020; 28 (3) : 341-350 Expression of FOXM1 and Aurora-A predicts prognosis and sorafenib efficacy in patients with hepatocellular carcinoma.
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Submit a Reference

REVIEW

Survivin antibody Cat. No. GTX100052

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-Survivin antibody used in IHC (Paraffin sections) (IHC-P). GTX100052
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : breast cancer tissue
Fixation : EDTA buffer, 0.3°C
Primary Antibody : 1:100, 4°C, 16Hr
Anti-Survivin antibody used in Western Blot (WB). GTX100052
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Lane 1: PANC1, Lane 2: AsPC1, Lane 3: BxPC3
Submit a Review
Application Reference
Mikami M et al. Mol Cells 2022; 45 (12):886-895 RUNX1-Survivin Axis Is a Novel Therapeutic Target for Malignant Rhabdoid Tumors.
Hattori EY et al. Commun Biol 2022; 5 (1):939 A RUNX-targeted gene switch-off approach modulates the BIRC5/PIF1-p21 pathway and reduces glioblastoma growth in mice.
Application : WB
Reactivity : Human
Lin TY et al. Phytomedicine 2021; 80:153384 Functional proteomic analysis reveals that fungal immunomodulatory protein reduced expressions of heat shock proteins correlates to apoptosis in lung cancer cells.
Application : WB
Reactivity : Mouse
Hueng-Chuen Fan et al. Int J Mol Sci 2020; 21 (22) Dehydroxyhispolon Methyl Ether, A Hispolon Derivative, Inhibits WNT/β-Catenin Signaling to Elicit Human Colorectal Carcinoma Cell Apoptosis.
Application : WB
Reactivity : Human
Su WL et al. Cancer Biomark 2020; 28 (3):341-350 Expression of FOXM1 and Aurora-A predicts prognosis and sorafenib efficacy in patients with hepatocellular carcinoma.
Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
Lee HJ et al. Front Immunol 2019; 10:1659 The Upregulation of Toll-Like Receptor 3 via Autocrine IFN-帣 Signaling Drives the Senescence of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Through JAK1.
Application : WB
Reactivity : Human
Liu YH et al. Phytomedicine 2018; 46:184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Yeh YH et al. J Exp Clin Cancer Res 2018; 37 (1):70 inflammatory * activates hif-1α-mediated epithelial-to-mesenchymal transition via pi3k/akt/mtor pathway.
Application : WB
Reactivity : Human
Fu JH et al. Eur Rev Med Pharmacol Sci 2018; 22 (2):351-357 MiR-182 affects renal cancer cell proliferation, apoptosis, and invasion by regulating PI3K/AKT/mTOR signaling pathway.
Application : WB
Reactivity : Human
Lee WY et al. Int J Cancer 2017; 141 (9):1921-1931 * sensitizes kras-mutant non-small-cell lung cancer to * by targeting taz.
Application : WB
Reactivity : Human
Liu H et al. Eur Rev Med Pharmacol Sci 2017; 21 (14):3193-3199 Inhibition of miR-221 influences bladder cancer cell proliferation and apoptosis.
Application : WB
Reactivity : Human
Chang YF et al. Oncotarget 2015; 6 (1):85-100 WMJ-S-001, a novel aliphatic hydroxamate derivative, exhibits anti-angiogenic activities via Src-homology-2-domain-containing protein tyrosine phosphatase 1.
Application : WB
Reactivity : Human
Hsiao CJ et al. J Nat Prod 2014; 77 (4):758-65 Cephalochromin Induces G0/G1 Cell Cycle Arrest and Apoptosis in A549 Human Non-Small-Cell Lung Cancer Cells by Inflicting Mitochondrial Disruption.
Application : WB
Reactivity : Human
Chang HL et al. Biochim Biophys Acta 2013; 1830 (8):4053-64 Simvastatin induced HCT116 colorectal cancer cell apoptosis through p38MAPK-p53-survivin signaling cascade.
Application : WB
Reactivity : Human
Hsu YF et al. Biochim Biophys Acta 2012; 1820 (2):104-15 Trichostatin A and sirtinol suppressed survivin expression through AMPK and p38MAPK in HT29 colon cancer cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169