Sample (30 μg of whole cell lysate)
A:NIH-3T3
15% SDS PAGE
GTX100441 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 μg of whole cell lysate)
A:293T
15% SDS PAGE
GTX100441 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Survivin antibody detects Survivin protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Survivin antibody (GTX100441) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Survivin antibody detects Survivin protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Survivin antibody (GTX100441) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of Survivin protein from 293T whole cell extracts using 5 μg of Survivin antibody (GTX100441).
Western blot analysis was performed using Survivin antibody (GTX100441).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Survivin(GTX100441) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.