Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Syk antibody [N2C2], Internal (GTX100748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SYK antibody [N2C2], Internal detects SYK protein at cytoplasm in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
SYK antibody [N2C2], Internal (GTX100748) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SYK antibody detects SYK protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SYK antibody [N2C2], Internal detects SYK protein at cytosol on rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
SYK antibody [N2C2], Internal (GTX100748) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SYK antibody [N2C2], Internal detects SYK protein at cytoplasm in human endometrium by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrium.
SYK antibody [N2C2], Internal (GTX100748) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 μg of SYK antibody [N2C2] (GTX100748).
Western blot analysis was performed using SYK antibody [N2C2] (GTX100748).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunofluorescence analysis of methanol-fixed A431, using SYK(GTX100748) antibody at 1:200 dilution.

SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SYK antibody [N2C2], Internal detects SYK protein by western blot analysis.
A. 30 μg mouse BMDM (bone marrow-derived macrophage) cells
B. 30 μg mouse Syk null cells
10% SDS-PAGE
SYK antibody [N2C2], Internal (GTX100748) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.