Syk (phospho Tyr323) antibody

Cat. No. GTX12862
Cat. No.  GTX12862
Anti-Syk (phospho Tyr323) antibody used in Western Blot (WB). GTX12862
GTX12862 WB Image

WB (peptide competition) analysis of Jurkat cells treated with 10 mM H2O2 for 3 minutes (Lane 2-6) using GTX12862 Syk (phospho Tyr323) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with lambda phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.

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Anti-Syk (phospho Tyr323) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12862
GTX12862 ICC/IF Image

ICC/IF analysis of Jurkat cells treated with 100 uM H2O2 for 1 hour using GTX12862 Syk (phospho Tyr323) antibody. Panel e shows untreated cells with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutesDilution: 2 μg/ml

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Anti-Syk (phospho Tyr323) antibody used in Western Blot (WB). GTX12862
Anti-Syk (phospho Tyr323) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12862
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF
  • Reactivity
    Human, Mouse
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