Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000.

Synaptophysin antibody [GT2589] detects Synaptophysin protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Synaptophysin antibody [GT2589] detects Synaptophysin protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Synaptophysin antibody [GT2589] detects Synaptophysin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Synaptophysin stained by Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Synaptophysin antibody [GT2589] detects Synaptophysin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Synaptophysin stained by Synaptophysin antibody [GT2589] (GTX633972) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min