Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Syntaxin 4 antibody [N2C3] (GTX114806) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX114806 diluted at 1:3000