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Syntaxin 6 antibody [N1C3]

Anti-Syntaxin 6 antibody [N1C3] used in Western Blot (WB). GTX115375
Anti-Syntaxin 6 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115375
Anti-Syntaxin 6 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115375
Anti-Syntaxin 6 antibody [N1C3] used in Western Blot (WB). GTX115375

Cat. No. GTX115375

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Syntaxin 6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

A431 , mouse brain , rat brain

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Syntaxin 6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621410

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

syntaxin 6

Cellular Localization

Golgi apparatus membrane; Single-pass type IV membrane protein

Background

Involved in intracellular vesicle trafficking.

Database

Research Area

DATA IMAGES

Anti-Syntaxin 6 antibody [N1C3] used in Western Blot (WB). GTX115375

GTX115375 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX115375 diluted at 1:1000

Anti-Syntaxin 6 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115375

GTX115375 ICC/IF Image

Syntaxin 6 antibody [N1C3] detects Syntaxin 6 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Syntaxin 6 protein stained by Syntaxin 6 antibody [N1C3] (GTX115375) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Syntaxin 6 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX115375

GTX115375 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Syntaxin 6(GTX115375) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Syntaxin 6 antibody [N1C3] used in Western Blot (WB). GTX115375

GTX115375 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntaxin 6 antibody [N1C3] (GTX115375) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wu SY et al. Autophagy 2023; 19 (4) : 1239-1257 Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169