WB analysis of various samples using GTX48659 TAF1 antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : A431 whole cell lysate
Lane 6 : A549 whole cell lysate
Lane 7 : LNCap whole cell lysate
Lane 8 : Molt-4 whole cell lysate
Lane 9 : Ramos whole cell lysate
Lane 10 : Raji whole cell lysate
Lane 11 : A-172 whole cell lysate
Lane 12 : NIH-3T3 whole cell lysate
Loading : 35 μg
Dilution : 0.2 μg/mL

Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody (GTX48659) was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). ChIP was performed on fos promoters using 5 μg of TAF1 antibody(GTX48659) and an RNA PolII antibody.

Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody (GTX48659) was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). ChIP was performed on fos promoters using 5 μg of TAF1 antibody(GTX48659) and an RNA PolII antibody.