Sample (30 ug of whole cell lysate)
A: Neuro2A
B: GL261
7.5% SDS PAGE
GTX117615 diluted at 1:1000

TBR1 antibody [N2C1], Internal detects TBR1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TBR1 stained by TBR1 antibody [N2C1], Internal (GTX117615) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

TBR1 antibody [N2C1], Internal detects TBR1 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TBR1 antibody [N2C1], Internal (GTX117615) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min