NCI-H929 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody [HL1954] (GTX637791) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TCF3 / E2A antibody [HL1954] detects TCF3 / E2A protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TCF3 / E2A stained by TCF3 / E2A antibody [HL1954] (GTX637791) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody [HL1954] (GTX637791) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

TCF3 / E2A antibody [HL1954] detects TCF3 / E2A protein by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TCF3 / E2A stained by TCF3 / E2A antibody [HL1954] (GTX637791) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.