APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , BCL-1 , Raw264.7 , PC-12 , Rat2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS
Predict Reactivity
Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888198
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TAR DNA binding protein , ALS10 , TDP-43
Cellular Localization
Nucleus
Background
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX630196 WB Image
Wild-type (WT) and TARDBP knockout (KO) HAP1 cell extracts (50 Âμg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
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GTX630196 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX630196 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT225] (GTX630196) diluted at 1:500.
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GTX630196 IHC-P Image
TDP43 antibody [GT225] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TDP43 antibody [GT225] (GTX630196) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX630196 WB Image
TARDBP antibody [GT225] detects TARDBP protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 10 % SDS-PAGE TARDBP antibody [GT225] (GTX630196) dilution: 1:1000
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GTX630196 IHC-P Image
TDP43 antibody [GT225] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. TDP43 antibody [GT225] (GTX630196) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX630196 WB Image
TARDBP antibody [GT225] detects TARDBP protein by western blot analysis. A. 30 μg BCL-1 whole cell lysate/extract B. 30 μg Raw264.7 whole cell lysate/extract 10 % SDS-PAGE TARDBP antibody [GT225] (GTX630196) dilution: 1:1000
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GTX630196 WB Image
TARDBP antibody [GT225] validation by siRNA knock-down. Upperpanel: TARDBP antibody [GT225] GTX630196 Lower panel: GAPDH antibody (GTX100118) A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells 10 % SDS-PAGE TARDBP antibody [GT225] (GTX630196) dilution: 1:3000 GAPDH antibody (GTX100118) dilution: 1:10000
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REFERENCE
REVIEW
TDP43 antibody [GT225]
Cat. No. GTX630196
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 26-Jul-2022
Application Tested :
WB
Sample Species :
Hu
Sample :
HAP1 WT and TARDBP KO cells
Amount used :
50μg
Blocking :
5% milk, 1 hr
Primary Antibody :
1:500 in 5% BSA and TBST, 4°C, > 12Hr
Figure Note :
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.