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TET3 antibody [HL2463]

Recombinant antibody KO/KD Overexpression
Anti-TET3 antibody [HL2463] used in Western Blot (WB). GTX638781
Anti-TET3 antibody [HL2463] used in Western Blot (WB). GTX638781
Anti-TET3 antibody [HL2463] used in IHC (Paraffin sections) (IHC-P). GTX638781
Anti-TET3 antibody [HL2463] used in IHC (Paraffin sections) (IHC-P). GTX638781
Anti-TET3 antibody [HL2463] used in IHC (Paraffin sections) (IHC-P). GTX638781

Cat. No. GTX638781

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2463

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TET3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

179 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human TET3.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tet methylcytosine dioxygenase 3 , hCG_40738

Cellular Localization

Nucleus,Cytoplasm

Background

Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-TET3 antibody [HL2463] used in Western Blot (WB). GTX638781

GTX638781 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [HL2463] (GTX638781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TET3 antibody [HL2463] used in Western Blot (WB). GTX638781

GTX638781 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [HL2463] (GTX638781) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET3 antibody [HL2463] used in IHC (Paraffin sections) (IHC-P). GTX638781

GTX638781 IHC-P Image

TET3 antibody [HL2463] detects TET3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TET3 stained by TET3 antibody [HL2463] (GTX638781) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET3 antibody [HL2463] used in IHC (Paraffin sections) (IHC-P). GTX638781

GTX638781 IHC-P Image

TET3 antibody [HL2463] detects TET3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
TET3 stained by TET3 antibody [HL2463] (GTX638781) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET3 antibody [HL2463] used in IHC (Paraffin sections) (IHC-P). GTX638781

GTX638781 IHC-P Image

TET3 antibody [HL2463] detects TET3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
TET3 stained by TET3 antibody [HL2463] (GTX638781) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations