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TFEB antibody [HL1065]

Recombinant antibody Comparison Orthogonal
Anti-TFEB antibody [HL1065] used in Western Blot (WB). GTX636089
Anti-TFEB antibody [HL1065] used in IHC (Paraffin sections) (IHC-P). GTX636089
Anti-TFEB antibody [HL1065] used in Western Blot (WB). GTX636089

Cat. No. GTX636089

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1065

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TFEB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TFEB. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909942

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription factor EB , ALPHATFEB , BHLHE35 , TCFEB

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-TFEB antibody [HL1065] used in Western Blot (WB). GTX636089

GTX636089 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with TFEB antibody [HL1065] (GTX636089) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TFEB antibody [HL1065] used in IHC (Paraffin sections) (IHC-P). GTX636089

GTX636089 IHC-P Image

TFEB antibody [HL1065] detects TFEB protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
TFEB stained by TFEB antibody [HL1065] (GTX636089) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TFEB antibody [HL1065] used in Western Blot (WB). GTX636089

GTX636089 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TFEB antibody [HL1065] (GTX636089) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations