Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TFF1 antibody (GTX121461) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of methanol-fixed MCF-7, using Trefoil factor 1(GTX121461) antibody at 1:500 dilution.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TFF1 antibody (GTX121461) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Trefoil factor 1 antibody detects Trefoil factor 1 protein at cytoplasm in mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Trefoil factor 1 antibody (GTX121461) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Trefoil factor 1(GTX121461) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min