HeLa whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N2C3] (GTX113723) diluted at 1:1000 and competitor's antibody (sc-274) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N2C3] (GTX113723) diluted at 1:1000 and competitor's antibody (sc-274) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.