TFIIE beta antibody immunoprecipitates TFIIE beta protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A : 30 μg whole cell lysate/extract of TFIIE beta protein expressing Jurkat cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of TFIIE beta by 2.5 μg of TFIIE beta antibody (GTX105029) 12% SDS-PAGE The immunoprecipitated TFIIE beta protein was detected by TFIIE beta antibody (GTX105029) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Sample (30 ug of whole cell lysate)
A: Jurkat nucleus
10% SDS PAGE
GTX105029 diluted at 1:5000

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-TFIIE beta antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.