Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-TFIIF antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX114455 diluted at 1:3000

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
7.5% SDS PAGE
GTX114455 diluted at 1:3000

TFIIF antibody immunoprecipitates TFIIF protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of TFIIF protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of TFIIF by 2.5 μg of TFIIF antibody (GTX114455) 7.5% SDS-PAGE The immunoprecipitated TFIIF protein was detected by TFIIF antibody (GTX114455) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.