Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with TGF beta 1 antibody (GTX130023) diluted at 1:500 and competitor's antibody (CST#3709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with TGF beta 1 antibody (GTX130023) diluted at 1:500 and competitor's antibody (#3709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TGF beta 1 antibody (GTX130023) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TGF beta 1 antibody (GTX130023) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.