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TGF beta 1 antibody

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX45121
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX45121
Anti-TGF beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX45121

Cat. No. GTX45121

Reference 13

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all TGF beta 1 products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: WB: 1:1000-1:10000.
For IHC: Use at a dilution of 1:200 - 1:500.
Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

44 kDa. ( Note )

Positive Control

TGFB1-transfected 293T

Product Note

TGFB1 detected in RAW264.7 cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

Recombinant protein - pig TGFB1

Purification

Protein A affinity purified
From polyclonal serum

Conjugation

Unconjugated

RRID

AB_1080946

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX45121

GTX45121 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TGF beta 1 antibody (GTX45121) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX45121

GTX45121 WB Image

TGF beta1 detected by western blot in RAW264.7 cells using anti-TGFB1 antibody (GTX45121)

Anti-TGF beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX45121

GTX45121 IHC-P Image

The data was published in the 2022 in Toxics. PMID: 35622687

REFERENCE

Application Reference

Garc?a-Arroyo FE et al. Antioxidants (Basel) 2023; 12 (5) Osthole Prevents Heart Damage Induced by Diet-Induced Metabolic Syndrome: Role of Fructokinase (KHK).
Alhanouf A Aljohani et al. Toxics 2022; 10 (5) The Anti-Rheumatic Drug, Leflunomide, Induces Nephrotoxicity in Mice via Upregulation of TGFβ-Mediated p53/Smad2/3 Signaling.
Han Wang et al. Mol Med Rep 2021; 24 (1) Transforming growth factor?β1 functions as a competitive endogenous RNA that ameliorates intracranial hemorrhage injury by sponging microRNA?93?5p.
Application : WB
Reactivity : Human
Dou B et al. Technol Cancer Res Treat 2020; 19 : 1533033820965580 Oncogenic Long Noncoding RNA DARS-AS1 in Childhood Acute Myeloid Leukemia by Binding to microRNA-425.
Ma C et al. Evid Based Complement Alternat Med 2020; 2020 : 4675851 Radix Angelica Sinensis and Radix Hedysari Ultrafiltration Extract Protects against X-Irradiation-Induced Cardiac Fibrosis in Rats.
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REVIEW

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Application Reference
Garc?a-Arroyo FE et al. Antioxidants (Basel) 2023; 12 (5) Osthole Prevents Heart Damage Induced by Diet-Induced Metabolic Syndrome: Role of Fructokinase (KHK).
Alhanouf A Aljohani et al. Toxics 2022; 10 (5) The Anti-Rheumatic Drug, Leflunomide, Induces Nephrotoxicity in Mice via Upregulation of TGFβ-Mediated p53/Smad2/3 Signaling.
Han Wang et al. Mol Med Rep 2021; 24 (1) Transforming growth factor?β1 functions as a competitive endogenous RNA that ameliorates intracranial hemorrhage injury by sponging microRNA?93?5p.
Application : WB
Reactivity : Human
Dou B et al. Technol Cancer Res Treat 2020; 19:1533033820965580 Oncogenic Long Noncoding RNA DARS-AS1 in Childhood Acute Myeloid Leukemia by Binding to microRNA-425.
Ma C et al. Evid Based Complement Alternat Med 2020; 2020:4675851 Radix Angelica Sinensis and Radix Hedysari Ultrafiltration Extract Protects against X-Irradiation-Induced Cardiac Fibrosis in Rats.
Chen WY et al. Cell Mol Immunol 2018; (Epub) Human dendritic cell-specific ICAM-3-grabbing non-integrin downstream signaling alleviates renal fibrosis via Raf-1 activation in systemic candidiasis.
Application : IHC-Fr
Reactivity : Mouse
Matsui F et al. J Gastroenterol 2018; (Epub) Induction of PIR-A/B+ DCs in the in vitro inflammatory condition and their immunoregulatory function.
Reactivity : Mouse
Chen PC et al. J Cell Physiol 2017; (Epub) Activation of Fibroblasts by Nicotine Promotes the Epithelial-Mesenchymal Transition and Motility of Breast Cancer Cells.
Application : WB
Reactivity : Human
Chuang YC et al. Neurourol Urodyn 2017; (Epub) Urodynamic and molecular characteristics of detrusor underactivity in a rat cryoinjury model and effects of low energy shock wave therapy.
Application : WB
Reactivity : Rat
Troyer RM et al. Exp Cell Res 2017; Exosomes from Osteosarcoma and normal osteoblast differ in proteomic cargo and immunomodulatory effects on T cells.
Application : WB
Reactivity : Human
Soldano S et al. PLoS One 2016; 11 (11):e0166433 Alternatively Activated (M2) Macrophage Phenotype Is Inducible by Endothelin-1 in Cultured Human Macrophages.
Application : WB
Reactivity : Human
Palmieri D et al. Adv Med Sci 2015; 60 (2):287-293 Effects of Pleiotrophin on endothelial and inflammatory cells: Pro-angiogenic and anti-inflammatory properties and potential role for vascular bio-prosthesis endothelialization.
Application : WB
Reactivity : Human
Garimella R et al. Transl Oncol 2014; 7 (3):331-40 Extracellular Membrane Vesicles Derived from 143B Osteosarcoma Cells Contain Pro-Osteoclastogenic Cargo: A Novel Communication Mechanism in Osteosarcoma Bone Microenvironment.
Application : WB
Reactivity : Human
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399