Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with THOC7 antibody (GTX119877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX119877 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed A431, using THOC7(GTX119877) antibody at 1:200 dilution.