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TLR7 antibody

Anti-TLR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX13730
Anti-TLR7 antibody used in Flow cytometry (FACS). GTX13730
Anti-TLR7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13730
Anti-TLR7 antibody used in Western Blot (WB). GTX13730
Anti-TLR7 antibody used in Flow cytometry (FACS). GTX13730
Anti-TLR7 antibody used in Flow cytometry (FACS). GTX13730
Anti-TLR7 antibody used in Flow cytometry (FACS). GTX13730
Anti-TLR7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13730

Cat. No. GTX13730

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all TLR7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 3 μg/ml
ICC/IF 1:5 - 1:1000
IHC-P Assay dependent
IHC-Fr 1:5 - 1:1000
FACS 2 - 5 μg / 10⁶ cells
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 706-749 of human TLR7; GenBank no. gb|AAF78035.1|AF245702_1. It will cross-react with mouse TLR7. In human Ramos cells, additional bands are seen.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor 7 , TLR7-like

Cellular Localization

Endoplasmic reticulum membrane,Endosome,Lysosome

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TLR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX13730

GTX13730 IHC-P Image

IHC-P analysis of human colon tissue using GTX13730 TLR7 antibody.
TOP : primary antibody
Bottom : isotype control
Dilution : 5 μg/ml

Anti-TLR7 antibody used in Flow cytometry (FACS). GTX13730

GTX13730 FACS Image

FACS analysis of mouse splenocytes using GTX13730 TLR7 antibody.
Red : primary antibody
Green : isotype control

Anti-TLR7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13730

GTX13730 ICC/IF Image

ICC/IF analysis of Ramos cells using GTX13730 TLR7 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:50
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-TLR7 antibody used in Western Blot (WB). GTX13730

GTX13730 WB Image

WB analysis of RAW264.7 cell lysate using GTX13730 TLR7 antibody.

Anti-TLR7 antibody used in Flow cytometry (FACS). GTX13730

GTX13730 FACS Image

FACS (Intracellular staining) analysis of human peripheral blood mononuclear cells (PBMCs) using GTX13730 TLR7 antibody.
Red : primary antibody
Green : isotype control

Anti-TLR7 antibody used in Flow cytometry (FACS). GTX13730

GTX13730 FACS Image

FACS analysis of human peripheral blood mononuclear cell (PBMC) using GTX13730 TLR7 antibody.
Red : Primary antibody
Green : isotype control
Shaded histogram : cell only
Dilution : 0.5 μg/ml

Anti-TLR7 antibody used in Flow cytometry (FACS). GTX13730

GTX13730 FACS Image

FACS (Intracellular staining) analysis of human monocyte using GTX13730 TLR7 antibody.
Red : Primary antibody
Green : isotype control
Shaded histogram : cell only
Dilution : 0.5 μg/10⁶ cells

Anti-TLR7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX13730

GTX13730 ICC/IF Image

ICC/IF analysis of RAW264.7 cells using GTX13730 TLR7 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:10

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399