Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TLR9 antibody [N1N2], N-term (GTX111547) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

TLR9 antibody [N1N2], N-term detects TLR9 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TLR9 stained by TLR9 antibody [N1N2], N-term (GTX111547) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TLR9 antibody [N1N2], N-term (GTX111547) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

TLR9 antibody [N1N2], N-term detects TLR9 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: TLR9 protein stained by TLR9 antibody [N1N2], N-term (GTX111547) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

TLR9 antibody [N1N2], N-term detects TLR9 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
TLR9 stained by TLR9 antibody [N1N2], N-term (GTX111547) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 μg of whole cell lysate)
A: Non-transfected 293T lysates
B: TLR9 transfected 293T lysates
7.5% SDS PAGE
GTX111547 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TLR9 antibody [N1N2], N-term detects TLR9 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
TLR9 stained by TLR9 antibody [N1N2], N-term (GTX111547) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min