The GTX14696 is supplied as a ready‐to‐use solution. Peroxidase reacts with the substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620‐650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths.
A soluble yellow product develops after stopping and stabilizing with a 450 nm stop solution.
The developing soluble blue product can be stopped and stabilized by using a 650 nm stop solution.