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TMEM119 antibody [HL2627]

Recombinant antibody Overexpression
Anti-TMEM119 antibody [HL2627] used in Western Blot (WB). GTX639083
Anti-TMEM119 antibody [HL2627] used in Western Blot (WB). GTX639083
Anti-TMEM119 antibody [HL2627] used in IHC (Paraffin sections) (IHC-P). GTX639083

Cat. No. GTX639083

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2627

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TMEM119 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:50000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Product Note

This antibody was raised against mouse TMEM119 Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of mouse TMEM119. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transmembrane protein 119 , AW208946 , BC025600 , obif

Cellular Localization

Cell membrane,Cytoplasm,Endoplasmic reticulum membrane

Background

Involved in several processes, including endochondral ossification; negative regulation of bone resorption; and positive regulation of macromolecule metabolic process. Acts upstream of or within osteoblast differentiation. Located in endoplasmic reticulum and plasma membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; limb; and skeleton. Orthologous to human TMEM119 (transmembrane protein 119). [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-TMEM119 antibody [HL2627] used in Western Blot (WB). GTX639083

GTX639083 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TMEM119 antibody [HL2627] (GTX639083) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TMEM119 antibody [HL2627] used in Western Blot (WB). GTX639083

GTX639083 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TMEM119 antibody [HL2627] (GTX639083) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TMEM119 antibody [HL2627] used in IHC (Paraffin sections) (IHC-P). GTX639083

GTX639083 IHC-P Image

TMEM119 antibody [HL2627] detects TMEM119 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TMEM119 stained by TMEM119 antibody [HL2627] (GTX639083) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations