PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, the pH 9.0 showed a strongest staining pattern. The pH 7.8 is acceptable but lower pH results in a significant reduction of sensitivity.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Appendix: An at least moderate, predominantly membranous staining should be seen in Cajal cells in the muscular wall and a weak membranous staining should be seen in columnar epithelial cells in the basal part of the crypts of the appendix.
Negative Control
Appendix: Staining should be absent in the majority of cells in the muscular wall and in epithelial cells located at the surface.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human DOG-1 protein fragment (aa 2-101)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
anoctamin 1 , DOG1 , ORAOV2 , TAOS2 , TMEM16A
Cellular Localization
Cell membrane , Cytoplasm
Background
Enables calcium activated cation channel activity; intracellular calcium activated chloride channel activity; and iodide transmembrane transporter activity. Involved in cation transport; inorganic anion transport; and positive regulation of insulin secretion involved in cellular response to glucose stimulus. Located in apical plasma membrane and nucleoplasm. [provided by Alliance of Genome Resources, Apr 2022]
Database
Research Area
DATA IMAGES
![Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369](/templates/default/js/lightbox/images/loading.gif "Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369")
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GTX04369 IHC-P Image
IHC-P analysis of human seminal vesicle tissue section using GTX04369 TMEM16A antibody [MSVA-201M] HistoMAX™.
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GTX04369 IHC-P Image
IHC-P analysis of human parotid gland tissue section using GTX04369 TMEM16A antibody [MSVA-201M] HistoMAX™.
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GTX04369 IHC-P Image
IHC-P analysis of human gastrointestinal stromal tumor tissue section using GTX04369 TMEM16A antibody [MSVA-201M] HistoMAX™.
Tumor periphery of a GIST showing intensive TMEM16A staining in all tumor cells.
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REFERENCE
There are currently no references for TMEM16A antibody [MSVA-201M] HistoMAX™ (GTX04369). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TMEM16A antibody [MSVA-201M] HistoMAX™ (GTX04369). Be the first to share your experience with this product.
![Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369](/upload/website/prouct_img/small/GTX04369/GTX04369_20230728_IHC-P_177_23072723_251.webp "Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369")
![Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369](/upload/website/prouct_img/small/GTX04369/GTX04369_20230728_IHC-P_307_23072723_341.webp "Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369")
![Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369](/upload/website/prouct_img/small/GTX04369/GTX04369_20230728_IHC-P_47_23072722_384.webp "Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369")
![Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369](/upload/website/prouct_img/normal/GTX04369/GTX04369_20230728_IHC-P_177_23072723_251.webp "Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369")
![Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369](/upload/website/prouct_img/normal/GTX04369/GTX04369_20230728_IHC-P_307_23072723_341.webp "Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369")
![Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369](/upload/website/prouct_img/normal/GTX04369/GTX04369_20230728_IHC-P_47_23072722_384.webp "Anti-TMEM16A antibody [MSVA-201M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04369")