Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, mouse TNF-alpha Protein (209.42-3.27 nM). Coated protein was probed with TNF alpha antibody [HL2363] (GTX638569) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Sandwich ELISA detection of recombinant 293T expressed, human Fc-tagged TNF alpha (54-233 a.a.) using antibodies as below.

Capture: TNF alpha antibody [HL2361] (GTX638567) (5 μg/mL)
Detection: HRP-conjugated TNF alpha antibody [HL2363] (GTX638569) (1 μg/mL)
Please notice that GTX638569 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638567. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of recombinant 293T expressed, human Fc-tagged TNF alpha (54-233 a.a.) using antibodies as below.

Capture: TNF alpha antibody [HL2363] (GTX638569) (5 μg/mL)
Detection: HRP-conjugated TNF alpha antibody [HL2361] (GTX638567) (1 μg/mL)
Please notice that GTX638567 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638569. Please contact us for custom HRP-conjugated antibody.