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TOMM20 antibody [GT1136]

Recombinant antibody
Anti-TOMM20 antibody [GT1136] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00773
Anti-TOMM20 antibody [GT1136] used in Western Blot (WB). GTX00773
Anti-TOMM20 antibody [GT1136] used in IHC (Paraffin sections) (IHC-P). GTX00773
Anti-TOMM20 antibody [GT1136] used in IHC (Paraffin sections) (IHC-P). GTX00773
Anti-TOMM20 antibody [GT1136] used in Immunoprecipitation (IP). GTX00773
Anti-TOMM20 antibody [GT1136] used in IHC (Paraffin sections) (IHC-P). GTX00773
Anti-TOMM20 antibody [GT1136] used in IHC (Paraffin sections) (IHC-P). GTX00773
Anti-TOMM20 antibody [GT1136] used in Western Blot (WB). GTX00773
Anti-TOMM20 antibody [GT1136] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00773
Anti-TOMM20 antibody [GT1136] used in Western Blot (WB). GTX00773

Cat. No. GTX00773

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1136

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all TOMM20 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:50000
ICC/IF 1:50 - 1:1000
IHC-P 1:50 - 1:1000
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of hmuan TOM20 (NP_055580.1)

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

translocase of outer mitochondrial membrane 20 , MAS20 , MOM19 , TOM20

Cellular Localization

Mitochondrion outer membrane

Database

Research Area

DATA IMAGES

Anti-TOMM20 antibody [GT1136] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00773

GTX00773 ICC/IF Image

TOMM20 antibody [GT1136] detects TOMM20 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TOMM20 stained by TOMM20 antibody [GT1136] (GTX00773) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-TOMM20 antibody [GT1136] used in Western Blot (WB). GTX00773

GTX00773 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TOMM20 antibody [GT1136] (GTX00773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TOMM20 antibody [GT1136] used in IHC (Paraffin sections) (IHC-P). GTX00773

GTX00773 IHC-P Image

IHC-P analysis of rat kidney tissue section using GTX00773 TOMM20 antibody [GT1136].
Dlution : 1:200

Anti-TOMM20 antibody [GT1136] used in IHC (Paraffin sections) (IHC-P). GTX00773

GTX00773 IHC-P Image

IHC-P analysis of hmuan colon carcinoma tissue section using GTX00773 TOMM20 antibody [GT1136].
Dlution : 1:200

Anti-TOMM20 antibody [GT1136] used in Immunoprecipitation (IP). GTX00773

GTX00773 IP Image

IP analysis of HeLa whole cell lysate (200μg) using GTX00773 TOMM20 antibody [GT1136].
IP antibody: 3μg
IB dilution : 1:1000

Anti-TOMM20 antibody [GT1136] used in IHC (Paraffin sections) (IHC-P). GTX00773

GTX00773 IHC-P Image

IHC-P analysis of hmuan colon carcinoma tissue section using GTX00773 TOMM20 antibody [GT1136].
Dlution : 1:400-1:3200

Anti-TOMM20 antibody [GT1136] used in IHC (Paraffin sections) (IHC-P). GTX00773

GTX00773 IHC-P Image

IHC-P analysis of mouse heart tissue section using GTX00773 TOMM20 antibody [GT1136].
Dlution : 1:200

Anti-TOMM20 antibody [GT1136] used in Western Blot (WB). GTX00773

GTX00773 WB Image

WB analysis of THP-1 whole cell lysate using GTX00773 TOMM20 antibody [GT1136].
Dilution : 1:10000-1:1280000
Loading : 25μg

Anti-TOMM20 antibody [GT1136] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00773

GTX00773 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00773 TOMM20 antibody [GT1136].
Orange: Primary antibody
Blue: DAPI
Dlution : 1:100-1:1600

Anti-TOMM20 antibody [GT1136] used in Western Blot (WB). GTX00773

GTX00773 WB Image

WB analysis of various samples using GTX00773 TOMM20 antibody [GT1136].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399