Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with TRADD antibody [N2C3] (GTX113042) diluted at 1:500 and competitor's antibody (sc-7868) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TRADD antibody [N2C3] detects TRADD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
TRADD stained by TRADD antibody [N2C3] (GTX113042) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min