Wild-type (WT) and TRAP1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRAP1 antibody [N1N3] (GTX115352) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TRAP1 antibody [N1N3] detects TRAP1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min and permeabilized with 100% MeOH for 30 sec.
Green: TRAP1 protein stained by TRAP1 antibody [N1N3] (GTX115352) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

TRAP1 antibody [N1N3] detects TRAP1 protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
TRAP1 antibody [N1N3] (GTX115352) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX115352 diluted at 1:3000