Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM27(GTX102098) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody (GTX102098) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using ret finger protein(GTX102098) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min