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TRIM72 antibody [HL1853]

Recombinant antibody Orthogonal
Anti-TRIM72 antibody [HL1853] used in Western Blot (WB). GTX637578
Anti-TRIM72 antibody [HL1853] used in Western Blot (WB). GTX637578
Anti-TRIM72 antibody [HL1853] used in Western Blot (WB). GTX637578
Anti-TRIM72 antibody [HL1853] used in Western Blot (WB). GTX637578
Anti-TRIM72 antibody [HL1853] used in IHC (Paraffin sections) (IHC-P). GTX637578
Anti-TRIM72 antibody [HL1853] used in IHC (Paraffin sections) (IHC-P). GTX637578

Cat. No. GTX637578

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1853

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TRIM72 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human TRIM72.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 72 , MG53

Cellular Localization

Cell membrane

Background

Enables identical protein binding activity. Predicted to be involved in several processes, including cellular protein metabolic process; plasma membrane repair; and protein homooligomerization. Predicted to act upstream of or within negative regulation of insulin receptor signaling pathway; negative regulation of insulin-like growth factor receptor signaling pathway; and negative regulation of myotube differentiation. Predicted to be located in cytoplasmic vesicle membrane. Predicted to be active in cytoplasm and sarcolemma. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-TRIM72 antibody [HL1853] used in Western Blot (WB). GTX637578

GTX637578 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM72 antibody [HL1853] (GTX637578) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRIM72 antibody [HL1853] used in Western Blot (WB). GTX637578

GTX637578 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TRIM72 antibody [HL1853] (GTX637578) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-TRIM72 antibody [HL1853] used in Western Blot (WB). GTX637578

GTX637578 WB Image

Orthogonal

Various whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TRIM72 antibody [HL1853] (GTX637578) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRIM72 antibody [HL1853] used in Western Blot (WB). GTX637578

GTX637578 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TRIM72 antibody [HL1853] (GTX637578) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRIM72 antibody [HL1853] used in IHC (Paraffin sections) (IHC-P). GTX637578

GTX637578 IHC-P Image

TRIM72 antibody [HL1853] detects TRIM72 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
TRIM72 stained by TRIM72 antibody [HL1853] (GTX637578) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TRIM72 antibody [HL1853] used in IHC (Paraffin sections) (IHC-P). GTX637578

GTX637578 IHC-P Image

TRIM72 antibody [HL1853] detects TRIM72 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
TRIM72 stained by TRIM72 antibody [HL1853] (GTX637578) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations