ICC/IF analysis of PFA-fixed C6 cells using GTX54860 TRPC3 antibody.
Panel A : Primary antibody
Panel B : DNA dye Hoechst 33342
Panel C : Merged images of panels A and B
Dilution : 1:500

IHC-Fr analysis of rat cerebellum tissue using GTX54860 TRPC3 antibody. DAPI is used as the counterstain (blue).
Panel A : TRPC6 (green) appears in molecular layer and in Purkinje cells.
Panel B : In the same section, staining of TRPC3 (red) appears as well in both molecular layer and Purkinje cells.
Panel C : Merge images of A and B indicates extensive co-localization.

IHC-P analysis of mouse cerebellum tissue using GTX54860 TRPC3 antibody.
Panel A : Primary antibody
Panel B : Antibody preincubated with the control peptide antigen

IHC-P analysis of rat pituitary gland tissue using GTX54860 TRPC3 antibody. TRPC3 is mainly expressed in the adenohypophysis (on left). Hematoxilin is used as the counterstain.
Dilution : 1:100

WB analysis of rat heart membrane lysate using GTX54860 TRPC3 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

IHC-Fr analysis of rat cerebellum tissue using GTX54860 TRPC3 antibody. DAPI is used as the counterstain (blue).
Panel A : TRPC3 (green) appears in Purkinje cells (arrows) including both soma and dendrites and as well as in the molecular layer neuropil (asterisk). Staining of the same section with mouse anti-parvalbumin (red) reveals that TRPC3 is not expressed in molecular layer interneurons.
Panel B : Pre-incubating Anti-TRPC3 with the peptide antigen