HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with TRPM2 antibody [N1N2-2], N-term (GTX112252) diluted at 1:500 and competitor's antibody (ACC-043) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

TRPM2 antibody [N1N2-2], N-term detects TRPM2 protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
TRPM2 antibody [N1N2-2], N-term (GTX112252) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

HepG2 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TRPM2 antibody [N1N2-2], N-term (GTX112252) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using TRPM2(GTX112252) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min