Date :
Anonymous submitted on 26-Jul-2022
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
HAP1 WT and TSC2 KO cells
Blocking :
5% BSA, 5% goat serum and 0.01% Triton X-100 in PBS, 30min, RT
Fixation :
4% PFA in PBS, 15min, RT
Permeabilization /Antigen retrieval :
0.1% Triton X-100 in PBS, 10min, RT
Primary Antibody :
1:800, 4°C, > 12Hr
Lane Description :
WT cells: Yellow dashed line
KO cells: Magenta dashed line
Figure Note :
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.