Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TSPAN3 antibody (GTX106868) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Sample (50 ug of whole cell lysate)
A: mouse kidney
12% SDS PAGE
GTX106868 diluted at 1:1000

TSPAN3 antibody detects TSPAN3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: TSPAN3 protein stained by TSPAN3 antibody (GTX106868) diluted at 1:500.
Blue: Hoechst 33342 staining.

TSPAN3 antibody detects TSPAN3 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
TSPAN3 antibody (GTX106868) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min